PHYLOGENY OF SOME MIDDLE AMERICAN PITVIPERS BASED ON A CLADISTIC ANALYSIS OF MITOCHONDRIAL 12S AND 16S DNA SEQUENCE INFORMATION

The cladistic relationships of several Middle American pitvipers representing the genera Bothrops (sensu stricto), Bothriechis, Cerrophidion, Lachesis and Porthidium were determined using mitochondrial 12S and 16S DNA sequence information. Maximum parsimony analyses were performed using PAUP on aligned sequences that included published information for related taxa. Two sets of analyses were conducted: one disregarding gaps in the aligned matrix, and another with gaps treated as a fifth base. When gaps were excluded resolution declined, although the general arrangement of the taxa changed little. A consistent relationship was the grouping of ((Porthidium, Bothriechis) Lachesis). The placement of Lachesis, as nested within other bothropoid genera, is only partially supported by results of other authors. The arrangement of Crotalus, Bothrops and Cerrophidion was ambiguous when gaps were discounted. In both trees, Agkistrodon was basal to the New World forms. The remaining genera, Trimeresurus (Protobothrops), Vipera, Azemiops, and Coluber, were uniformly distant to the former taxa. Also of interest is the lack of close relationship, based on the DNA data here and elsewhere, between Bothrops and Porthidium. This is in striking contrast to results based on morphologic and allozymic analyses of previous studies. It is concluded that additional DNA sequence information from a larger sample of taxa will be necessary to better assess the phylogenetic relationships among Middle American and related pitvipers

Meeting The Needs Of Rural Site-Bound Paraeducators

The Elementary and Secondary Education Act 2002-No Child Left Behind (NCLB) mandates that paraeducators who work with today’s children and youth are highly qualified.  Montana State University-Billings (MSU-Billings) has revised its Associate of Arts Program of Study in Education with the intention of supporting Montana and other rural states in complying with this mandate.  In additioin to a two-year program of study at our main campus, the MSU-Billings College of Technology provides an exam for paraeducators with preparatory/remedial support modules

Why They Come, Why They Go, and Why They Stay: Factors Affecting Volunteerism in 4-H Programs

The 4-H program depends on the work of thousands of adult volunteers. It seems prudent to understand more about the factors involved in an adult becoming a leader, factors contributing to the leader having a positive experience, and, in particular, the factors causing the leader to end his or her role. In order to better understand the experience of 4-H leaders and the factors contributing to their success, an exit survey was conducted with individuals who had terminated their leadership role. Key reasons for volunteering and leaving are identified

Using Focus Groups to Evaluate Youth Development Program Direction

Focus groups are an efficient and effective assessment tool for youth development practitioners with limited time and resources. This article examines the practical use and limitations of focus groups to assess a county 4-H program\u27s response to a changing demographic profile. A complete description of the process is followed by a report of results and implications for program direction

Archaeological Survey of the Proposed Location for a Borrow Pit, West of Solsberry, Greene County, Indiana

Abstracts are made available for research purposes. To view the full report, please contact the staff of the Glenn A. Black Laboratory of Archaeology (www.gbl.indiana.edu).At the request of Duncan Robertson, Inc., the Glenn A. Black Laboratory of Archaeology, Indiana University (GBL) performed a cultural resources survey of the proposed location for excavation of a borrow pit west of Solsberry, Greene County, Indiana. A total of approximately 0.45 acres was surveyed. The purposes of the survey were 1) to identify and document all of the cultural resources in the project area, 2) to evaluate any sites with regard to their eligibility for inclusion on the National Register of Historic Places (NRHP) and the Indiana Register of Historic Sites and Structures (IRHSS), and 3) to make recommendations for the protection of significant and potentially significant sites. Fieldwork was conducted August 5, 1999 by GBL archaeologist Andrew A. White. No cultural materials were discovered in the project area. Cultural resource clearance is recommended for the project area provided that all earth-moving activities are restricted to the current proposed area of impact

Gas Accretion is Dominated by Warm Ionized Gas in Milky Way-Mass Galaxies at z ~ 0

We perform high-resolution hydrodynamic simulations of a Milky Way-mass galaxy in a fully cosmological setting using the adaptive mesh refinement code, Enzo, and study the kinematics of gas in the simulated galactic halo. We find that the gas inflow occurs mostly along filamentary structures in the halo. The warm-hot (10^5 K 10^6 K) ionized gases are found to dominate the overall mass accretion in the system (with dM/dt = 3-5 M_solar/yr) over a large range of distances, extending from the virial radius to the vicinity of the disk. Most of the inflowing gas (by mass) does not cool, and the small fraction that manages to cool does so primarily close to the galaxy (R <~ 20 kpc), perhaps comprising the neutral gas that may be detectable as, e.g., high-velocity clouds. The neutral clouds are embedded within larger, accreting filamentary flows, and represent only a small fraction of the total mass inflow rate. The inflowing gas has relatively low metallicity (Z/Z_solar < 0.2). The outer layers of the filamentary inflows are heated due to compression as they approach the disk. In addition to the inflow, we find high-velocity, metal-enriched outflows of hot gas driven by supernova feedback. Our results are consistent with observations of halo gas at low z.Comment: 10 pages including 5 figures, submitted to Ap

A Stellar Population Gradient in VII Zw 403 - Implications for the Formation of Blue Compact Dwarf Galaxies

We present evidence for the existence of an old stellar halo in the Blue Compact Dwarf galaxy VII Zw 403. VII Zw 403 is the first Blue Compact Dwarf galaxy for which a clear spatial segregation of the resolved stellar content into a "core-halo" structure is detected. Multicolor HST/WFPC2 observations indicate that active star formation occurs in the central region, but is strikingly absent at large radii. Instead, a globular-cluster-like red giant branch suggests the presence of an old (> 10 Gyr) and metal poor (=-1.92) stellar population in the halo. While the vast majority of Blue Compact Dwarf galaxies has been recognized to possess halos of red color in ground-based surface photometry, our observations of VII Zw 403 establish for the first time a direct correspondence between a red halo color and the presence of old, red giant stars. If the halos of Blue Compact Dwarf galaxies are all home to such ancient stellar populations, then the fossil record conflicts with delayed-formation scenarios for dwarfs.Comment: Accepted for publication in the Ap

Two closely related ureotelic fish species of the genus Alcolapia express different levels of ammonium transporters in gills

Most fish excrete their nitrogenous waste across the gills as ammonia through the activity of the Rhesus glycoprotein ammonium transporters. In contrast, fish of the subgenus Alcolapia (Oreochromis) are the only vertebrates that survive the extreme conditions of the soda lakes of Natron and Magadi in East Africa and have evolved adaptations to the highly alkaline waters including the ability to excrete their nitrogenous waste as urea. Nevertheless, Alcolapia retain the Rhesus glycoprotein genes in their genomes and using two heterologous expression systems, we demonstrate that Alcolapia Rhbg is capable of moving ammonia. Comparing ammonia and urea excretion from two closely related Alcolapia species from the same aquarium, we found that while Alcolapia grahami remains fully ureotelic after many generations in lab conditions, Alcolapia alcalica excretes some of its nitrogenous waste as ammonia. Using in situ hybridisation, we demonstrate robust, localised gene expression of Rhbg, rhcg1 and rhcg2 in the gill tissue in both A. alcalica embryos and adults, similar to that in other ammoniotelic fish. In contrast, the expression of these genes in A. grahami gills is much lower than in A. alcalica, suggesting the rapid evolution of a molecular mechanism underlying the complete ureotelism of A. grahami